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A bacteriophage transfers DNA of the previous host to the current host.This is an example of ________.


A) conjugation
B) generalized transduction
C) specialized transduction
D) a reaction of an Hfr cell
E) None of the choices are correct.

F) D) and E)
G) A) and B)

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In bacteria,initiator tRNA carries formyl methionine.

A) True
B) False

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A permanent,inheritable change in the genetic information is called ________.


A) translation
B) transcription
C) mutation
D) alteration
E) regeneration

F) B) and E)
G) A) and E)

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DNA is denatured at ________.


A) 37ᵒC
B) 42ᵒC
C) 60ᵒC
D) 94ᵒC
E) 100ᵒC

F) B) and C)
G) A) and B)

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After three replication cycles in PCR,there will be a total of three double-stranded DNA molecules.

A) True
B) False

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A 59-year-old female has been diagnosed with Escherichia coli infection following the consumption of contaminated meat. The patient is exhibiting a high fever, severe abdominal cramping, and diarrhea. The RN provides supportive care and fluid resuscitation as ordered. The nurse and medical team provide education to the patient and her family regarding the genetics of the E. coli bacterium. -The RN instructs the family that the transfer of genetic material can involve contact between two bacterial cells.DNA can pass from one cell to the other through a pilus during this process,which is called ________.


A) transformation
B) conjugation
C) specialized transduction
D) generalized transduction

E) A) and B)
F) C) and D)

Correct Answer

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DNA fragments can be separated in gel electrophoresis because ________.


A) nitrogenous bases have a net positive charge
B) nitrogenous bases have a net negative charge
C) phosphate groups have a net positive charge
D) phosphate groups have a net negative charge
E) None of the choices are correct

F) B) and C)
G) B) and D)

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Which of the following is incorrect about transfer RNA?


A) It has a bottom hairpin loop with an anticodon.
B) An anticodon is complementary to a codon.
C) It contains a binding site for an amino acid.
D) The initiator tRNA that binds to the P site has the anticodon UAC.
E) The initiator tRNA in bacteria carries tryptophan.

F) C) and E)
G) B) and D)

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Which of the following is not true of an organism's genotype?


A) Is inherited
B) Are structural genes coding for proteins
C) Are genes coding for RNA
D) Are regulatory genes controlling gene expression
E) Are the expressed traits governed by the genes

F) None of the above
G) A) and E)

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An 18-month-old male is admitted to the hospital with symptoms of a respiratory infection. The patient's mother reports that since birth, the toddler has frequently had a cough and copious nasal secretions. His weight gain has been poor, despite his excellent appetite. The patient appears in moderate respiratory distress, so oxygen therapy and intravenous fluids are initiated. The physician orders tests for cystic fibrosis due to the patient's history and clinical presentation. When the tests confirm the diagnosis, as the RN, you provide education to the mother regarding this autosomal recessive disease. -The RN is aware that many different types of mutations have been identified in the gene that causes cystic fibrosis.Which of the following terms would best describe a mutation that changes a normal codon into a stop codon,usually resulting in a nonfunctional protein? 


A) Missense mutation
B) Nonsense mutation
C) Point mutation
D) Frameshift mutation

E) A) and B)
F) A) and C)

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If the wild-type DNA sequence reads THE CAT ATE THE BIG RAT,what type of mutation would change the sequence to THE CAT ATE THE BAG RAT?


A) Missense
B) Nonsense
C) Insertion
D) Deletion
E) Silent

F) B) and E)
G) A) and B)

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Excised introns form structures called lariats.

A) True
B) False

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Repressible operons are normally turned on in the cell.

A) True
B) False

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A recombinant organism is one that has sustained a change in its genome through receipt of DNA from a different organism.

A) True
B) False

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The benefit of the bacterial operon is that ________.


A) since the proteins coded for by the operon structural genes work as a team,they are regulated (i.e.induced or repressed) as a team,thus saving energy
B) the structural genes can be regulated individually,affording the cell more control over the protein products
C) the split genes can be cut and spliced back together with greater precision than with single genes
D) they are much less prone to mutation than individual genes

E) A) and B)
F) None of the above

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Restriction endonucleases are obtained from various species of bacteria.

A) True
B) False

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All mutations are detrimental to the cell.

A) True
B) False

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The DNA of microorganisms is made up of subunits called ________.


A) histones
B) amino acids
C) nucleotides
D) mRNA
E) polymerases

F) A) and D)
G) C) and D)

Correct Answer

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If the wild-type DNA sequence reads THE CAT ATE THE BIG RAT,what type of mutation would change the sequence to THE CAT ATE (stop) ?


A) Missense
B) Nonsense
C) Insertion
D) Deletion
E) Silent

F) A) and E)
G) C) and D)

Correct Answer

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The process in which mutations are removed and the correct bases added is called ________.


A) transduction
B) excision repair
C) frameshift
D) back-mutation
E) transformation

F) B) and C)
G) C) and D)

Correct Answer

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